Importance Of Serial Dilution In Serology Crp License Open Access

Importance Of Serial Dilution In Serology Crp License Open Access

Davidson b Show more Add to Mendeley Share Cite Get rights and content Under a Creative Commons license open access Highlights We estimate microbial concentration from measured counts on a single agar plate.

Estimation is a function of dilution factor and ratio of plate size to colony size.

We give a procedure to select optimal dilution plate for serial dilution process.

We predict optimal number of colonies on agar plate that minimizes the total error.

Total error includes the competing effects of sampling and counting errors.

Abstract Titration of microorganisms in infectious or environmental samples is a corner stone of quantitative microbiology.

A simple method is presented to estimate the microbial counts obtained with the serial dilution technique for microorganisms that can grow on bacteriological media and develop into a colony.

The number (concentration) of viable microbial organisms is estimated from a single dilution plate (assay) without a need for replicate plates.

Our method selects the best agar plate with which to estimate the microbial counts, and takes into account the colony size and plate area that both contribute to the likelihood of miscounting the number of colonies on a plate.

The estimate of the optimal count given by our method can be used to narrow the search for the best (optimal) dilution plate and saves time.

The required inputs are the plate size, the microbial colony size, and the serial dilution factors.

The proposed approach shows relative accuracy well within 0.1 log 10 from data produced by computer simulations.

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